FV retrieved the sequences and performed the alignment assays.
In alignment assays, the clone C32006D10, that had been isolated from the cDNA library RVDevelop1, showed a high degree of sequence identity (75%) with the Arabidopsis gene SEP3.
Cell alignment was assayed after shear stress for 16 hr (n = 6).
SIFT Ng and Henikoff, 2006 and AlignGV-GD Tavtigian et al., 2006, for example, use multiple alignments to assay conservation levels of novel amino acid changing variants with the underlying assumption that sequence variants, which alter highly conserved positions in protein sequences are a priori more likely to be damaging.
Cell proliferation was examined by MTT assay, alignment of actin fibers by a designed image processing code, and cell differentiation by fluorescence staining.
ZJ and GH participated in the sequence alignment, RT-PCR assay and performed the statistical analysis.
Sequence alignments of the assay sequences to the GSS sequences of Brassica oleracea TO1000DH3 or TO1434 [ 14, 15] as well as WGS sequences of Brassica rapa Chiifu-401 [ 18] and Brassica oleracea O212 were also exploited to determine which of the assay nucleotides corresponded to the A and C genomes, respectively.
RepMP4 and RepMP2/3 genes of MP positive samples were amplified for molecular typing through sequence alignment and PCR-RFLP assay.
The fact that Rep/C4 repress CMT3 expression in the host is in perfect alignment with our ChIP assay results on CaLCuV-infected plants, where the loss of H3K9me2 is even more severe than the observed in TrAP transgenic plants.
Size exclusion chromatography (SEC), SDS-PAGE, microtubule assembly assays, aggregation assays, multiple sequence alignment, and molecular modeling characterized the dynamic response of tubulin assembly to increasing concentrations of αB crystallin.
