Alignments were manually adjusted as needed.
Kinase domains were identified by analysis of alignments with the Pfam protein kinase domain, and extended manually as needed.
Manual alignment adjustments were made as needed with the assistance of the BioEdit multiple alignment tool of Hall [ 55].
This method requires a long period of hospitalization and accurate control of fracture alignment with frequent radiographs and adjustments in traction as needed.
Preliminary multiple sequence alignments were constructed with Clustal W [ 51] or MUSCLE [ 52] and were trimmed and adjusted manually as needed.
If this alignment is unsatisfactory in your case, fine adjustments may be carried out by scaling, rotating, and translating the implant model as needed within 3-matic.
It could provide some cues for the development of novel implantable biomaterials, such as tissue-engineered scaffolds for cartilage replacement, where specific cell alignment is needed.
Users may thus jump between reference tabs to inspect outputs in detail (eg, individual sequence alignments when needed, not shown) as selected in the query options (Fig. 3).
The disadvantage of trCYK is that unlike local primary sequence alignment, which is as computationally efficient as global sequence alignment, it needs to track the three possible structural alignment modes (joint, left marginal and right marginal) in the dynamic programming recursion.
For both algorithms, we found that the 99% fastest alignments need nearly as much computing time as the remaining 1% slowest alignments.
The overall purpose of the process was to inform and influence the funding strategies of global level research funders, and to promote funding alignment with policy maker needs, as well as to stimulate more coordinated researcher interest in high priority questions.
