Sentence examples for alignment as necessary from inspiring English sources
If new gaps are introduced here, gaps are also inserted into the existing alignment as necessary.
Sequences were trimmed with Sequencher 4.7 (GeneCodes) to remove vector and ambiguous sequence and compared to cDNAs using contig assembly, blast, and manual alignments as necessary to define intron/exon boundaries, start and stop codons, splice sites and polyadenylation sites.
Expanded multiple sequence alignments were generated as necessary using the MEGA5 program (Tamura et al. 2011) and MUSCLE (Edgar 2004) after retrieval of additional sequences from the database used by Curtis et al. (2012) and from the NCBI GenBank nr (nonredundant) database.
Alignments were subsequently manually adjusted as necessary when sequences with variable-length helices and/or loops were added.
Alignments were built using NAST aligner [ 30] and imported into ARB and corrected as necessary.
The LTR alignments were verified using ClustalX [ 15], after manual editing as necessary (see Supplemental data).
Amino acid alignments were performed using ClustalW (version 1.8) software and adjusted as necessary [ 56].
Summed dynamic PET images were inspected for alignment with the corresponding attenuation correction CT images, and manually realigned and re-reconstructed as necessary.
The alignment box won't disappear until you decide to close it, so feel free to resize as much as necessary.
Recalibrate as necessary.
