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Domains were defined as individual introns or exons, recombination breakpoints were determined both by visual inspection of the alignment and use of RDP.
It does not make any sense to report all these results because we present an alignment and use the results of multiple searches to manually correct it.
The principle of CARMA is to align a sequence hitting a Pfam model with the model's curated seed alignment, construct a neighbor-joining tree from the alignment and use this tree to infer the sequence's taxonomy.
We couple the alignment module with a novel statistical model to assess the significance of each alignment and use it to infer the respective similarity/dissimilarity of human tissue-specific networks with their corresponding counterparts in yeast.
To calculate the difference between obtained estimates, we applied the approach proposed by Le and Gascuel (2008): Measure the Akaike information criterion, AIC (Akaike 1974) for each alignment and use the nonparametric paired sign test on the likelihood values, which are estimated per alignment site, to assess the significance of the difference between models.
Lastly, multiple sequence alignment (MSA) based methods such as Coral [ 11] and SEECER [ 12] cluster reads that share k-mers to create a local vicinity and a multiple alignment, and use the consensus sequence as a guide to correct the reads.
Similar(54)
Further all the MAPKKK sequences were aligned using CLUSTAL 2.0.3 (http://www.ebi.ac.uk/clustalw/) multiple sequence alignment and used to construct HMM profile.
So, using an Ilizarov frame, a scaffold around the leg that looks a bit like a medieval torture device, his treatment consisted of two main elements — returning the ankle joint to something near its normal alignment and using the body's ability to grow new bone to lengthen the leg to where it had been before the accident.
Moreover, many reports on the effect of cervical sagittal alignment did not designate the ideal definition of alignment and used different definitions of lordosis.
From this global alignment, and using the defining fingerprints of the different types (see below) [13], [17], the Gold Standard sequences were manually classified as members of each group.
A phylogenetic tree of the Tetrahymena MTs was constructed starting with the T-coffee alignment and using MrBayes software under a "mixed protein" model [82].
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