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Indeed, whichever program, query alignment and reference alignment we used, 13 HEAT repeats ( = 14%) and six ARM repeats ( = 10%) amongst the repeats from known structures remained undetected.
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We used true simulated reference alignments and reference trees with RAxML-estimated branch lengths.
Such tools should also enable users to display their data alongside canonical gene annotations, EST alignments and reference datasets harvested from the public domain.
Then, a manual gap joining stage used the layout of the contigs according to their aligned positions on the reference using the AMOS package and manual examination of each gap, adjacent contig alignments and reference annotation in the MAUVE visualization tool.
A number of realignment or assembly methods have been proposed to overcome the alignment errors and reference bias that limit indel detection.
All overlapping regions between alignments and referenced exons were counted.
We have specified formats for biological sequences, sequence annotations and alignments and references to data and resources.
BioXSD is represented by a dedicated XML Schema and defines syntax for biological sequences, sequence annotations, alignments and references to resources.
We previously validated that nuclear alignment is a suitable metric that is highly correlated with cell alignment and that reference is now cited (Baeyens, Mulligan-Kehoe et al., 2014).
CDD [ 46] provides a collection of PSSM profiles for Pfam families and MulPSSM [ 47] is another related resource that use multiple PSSMs corresponding to a given alignment and variable reference sequences.
Before the second alignment, the customized reference was updated to ensure that one allele was present at each heterozygous site predicted in the initial alignment, and non-reference alleles were added to the separate variant file.
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