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The computerized multiple tilt series collection scheme is implemented together with automated advanced procedures making collection, image alignment, and processing of multi-tilt tomography data a seamless process.
The comparison of MaSp1 and 2 films also shows that with despite similar alignment and processing, the MaSp2 films do not perform as well as MaSp1 films.
We thank Coleen Moloney and Cliff Dorse for assistance with collecting samples, Helena Westerdahl for providing primers, and Martin Stervander for alignment and processing of sequence data.
Section 2 describes the experimental method giving a description of the SS-OCT system, details on animal preparation, electrocoagulation technique, anatomical slice preparation and finally OCT image alignment and processing details.
The genome analysis pipeline is composed of three main components: a module that performs raw sequence alignment and processing, a module that performs custom CNV and SNP/LOH analyses, and a module that constructs figures summarizing all completed analyses and then displays them on the webpage.
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MPS and DB provided code to perform the alignment and reads processing.
Motion compensation, including range alignment and autofocus processing, is performed to remove the translational motion in advance.
We altered the alignment and alignment processing parameters to test the robustness of our analysis.
Ten nanometer gold fiducials were used for alignment, and all processing was carried out with the TOM toolbox.
Interferometry requires the use of multiple objective lenses, significantly increasing the complexity of the system and making alignment and data processing more challenging.
The actual extent and the root cause of these blind spots vary based on the aligner, the parameters used in the alignment and post-processing steps, and the types of the variants.
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