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For certain tasks of bioinformatics workflows, such as sequence alignment and mapping of short reads which are also incidentally some of the most computationally expensive processes, this approach can allow for a very high degree of parallelism in multiprocessor architectures and distributed computing environments.
Short read alignment and mapping to the reference genome.
For the alignment and mapping of sequencing reads, we used Bowtie.
BWA [ 16] was employed for the alignment and mapping of reads to the sheep genome.
In addition, user-friendly web tools like browsing, sequence alignment and mapping are also integrated into the NeuroPep database.
Read alignment and mapping was performed using programs BWA (0.7.3) and SAMtools (0.1.17) (Li and Durbin, 2009; Li et al., 2009) and BedTools (Quinlan and Hall, 2010).
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Three mutations are classified as structural variants (p.I298T, p.F318C, p.E383A), as they affect conserved positions in the repeat alignment and map into the space between the two β-sheets of repeats 2 and 3 (Figures 4 and 5).
VLS and AvH generated the sequence alignment and mapped the results for matK editing sites on the phylogenetic tree.
Sequence conservation was calculated from the sequence alignment and mapped onto the protein structure using ConSurf (Armon et al. 2001; Glaser et al. 2003).
Unassigned BACs, also referred to as chromosome 0 BACs, lack sequence alignment and mapped markers so they could not be reliably assembled into any of the pseudomolecules.
We therefore created an alignment of eukaryotic RNA polymerases, plotted the level of conservation in 10 amino-acid windows across the alignment, and mapped the intein insertion sites onto the plot.
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