The phrase "alignment and further" is not correct and may be unclear in written English.
It could be used in contexts where you are discussing alignment and additional actions or considerations, but it lacks clarity.
Example: "We need to focus on alignment and further steps to ensure project success."
Alternatives: "alignment and additional" or "alignment and next steps".
The image after segmentation has much cleaner signals for more precise alignment and further analysis.
For phylogenetic analyses, 46 outgroups and reference ITS sequences from Pezizomycetes (15), Orbilliomycetes (5), and three lichenized classes including Arthoniomycetes (15), Lichinomycetes (3), and Lecanoromycetes (8) were added to the data set for alignment and further analyses.
Clustering with BLASTCLUST followed by multiple sequence alignment and further sequence profile searches were used to identify other domains that were not present in the Pfam database.
After checking for Mendelian segregation and eliminating loci that deviated strongly, we visually inspected every parental alignment and further eliminated all loci that had more than two haplotypes per individual/contig.
Sequences were only included in both the alignment and further analysis if the QA region of the sequence contained no ambiguous trace file calls; thus the QA ratio is likely to be correct in these cases, even though the flanking sequence was partially truncated.
In addition, polymorphisms, reference sequence errors and sequencing errors require that mismatches and indels be allowed in read alignments and further contribute to lower our confidence in mappings.
The extraction, alignment, deconvolution, and further processing of raw GC/MS data were principally referred to the previous published protocol [ 35].
The extraction, alignment, deconvolution, and further processing of raw GC/MS data were referred to the protocols of literature [ 47- 49], except the mass window was set to 70 600 m/z.
To date, deep sequencing data has been analysed by applying various read quality filters before and after alignment and then further manually filtering the results, according to the researcher's understanding of the sequencing technology and the individual data set [ 16].
The sequence reads were mapped onto CEN.PK113-7D CEN.PK113-7D usingenomeows–Wheeler Alignment tool (BWA) and further processed using SAMtools [ 76- 78].
The sequence reads were mapped onto CEN.PK113-7D CEN.PK113-7DusingenomeBurrows-Wheeler Alignment tool BWA and further processed using SAMtools (69, 70).
