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However, we still used manually annotated eye coordinates for alignment and estimation of facial fiducial points for local feature extraction.
If manual markings were used for alignment and estimation of the facial fiducial points for local feature extraction, the proposed algorithm performs best.
This figure depicts the contribution of the false rejection rate (P FR) and the false classification rate (P FC) to the overall performance of the proposed system when automatically detected facial features were used for alignment and estimation of other locations for local feature extraction.
For alignment and estimation of genetic distance between sequences, we used BioEdit 7 [ 61].
Read alignment and estimation of transcript abundance was performed using Bowtie2 [ 44] and RSEM [ 45] as described in the Trinity abundance estimation protocol.
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One potential approach to this issue is simultaneous alignment and tree estimation.
Environmental sampling holds the key to many focal questions in mycology, and simultaneous alignment and tree estimation, as performed by SATé, can be a highly efficient companion in that pursuit.
One novel approach is simultaneous alignment and tree estimation, such as that performed by SATé, a recently released program [21] [22] that unites the inference of optimal multiple alignments and phylogenetic trees into a single process, thereby eliminating manual examination of MSAs for molecular ecological inference.
Fasta alignment and phylogeny estimation was carried out with MEGA5 [ 64].
The abundance of every gene (component) in each of the eight samples was determined by performing alignment and abundance estimation using the RSEM package [ 93] (version 1.2.3).
Finally, the percentage of reads mapping (methods described in Read alignment and abundance estimation) to this assembly was higher than the previous assembly (84% vs. 82% average per sample).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com