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Tew and Waugh found that properly aligned tibial components are the most successful, but proper alignment alone does not guarantee long-term success.
This change is larger than would be anticipated from enhanced calcium handling and increased uniaxial alignment alone.
This alignment alone is worth keeping an eye open for but starting on 7 March the waning gibbous moon joins the tableau, making it even more special.
However, Resnick and Shipley (2013) found that there might be an additive benefit for hierarchical and progressive alignment over progressive alignment alone.
However, optical momentum alignment alone is not enough to induce a net electric current due to the symmetrical distribution of carriers with momenta k and −k in optical excitation.
The coverage of MIPS was more than twice that of using sequence-based alignment alone.
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As stated in the manuscript, the authors emphasize that sequence alignments alone are unlikely to define alcohol-binding motifs in these proteins; instead, the conformational proximity of the appropriate amino acids is likely to be more relevant.
Furthermore, we also observed that 62 cases (5%) with sequence identities below 30% allowed for uni- or bi-lateral exchange, thereby making it extremely difficult to predict potential cross-reactions from sequence alignments alone.
Comparison of phylogenetic variation with sequences obtained by experiment (Table 1) shows that several amino acid substitutions in F0-GFP, including F27W, F46A, and the F83W/F84W combination, could not have been anticipated based on sequence alignments alone, similar to what was observed for the 57 residue β-barrel-like src SH3 domain [30], hence supporting a fully random mutagenesis approach.
Moreover, lncRNA elements tend to maintain a consensus secondary structure through compensatory base mutations and, therefore, are difficult to detect by sequence alignments alone [ 19].
This was also supported by combined indel data from ancestral sequence reconstruction as well as by the data derived from alignments alone (alignments R = 0.23, ancestral sequences R = 0.33).
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CEO of Professional Science Editing for Scientists @ prosciediting.com