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Strategies to align cores along a chromosome include the use of pedigree information (either explicitly or as part of a segregation analysis), using overlapping cores to step across a chromosome, or aligning based on the similarity between clusters of surrogates for adjacent cores.
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The corresponding nucleotide gene family datasets were aligning based on their protein alignments using in-house software.
In aligning time samples based on the inferred time lags, we assume that the time-series data are not periodic.
When aligning sequences, insertions/deletions are allocated based on the sequence context and strand orientation.
Some are expressed, based on the presence of an aligning EST, and have homologs in Mlp, suggesting an importance.
Following assembly, transcripts were assigned an RPKM [ 68] value based on the number of uniquely mapping reads aligning to each transcript using BWA [ 69].
In terms of the femur, the principle of aligning the varus/valgus osteotomy of the distal femur in TKA was based on the assumption that the angle formed by the anatomic and mechanical axes of the femur is consistently between 5° and 6° [ 15, 19, 31].
In brief, this was based on the algorithm of de Bruijn graph construction which began by aligning k-mers (k = 25), and reads with certain length of overlap were joined to form linear contigs.
We used a "best hit" criterion, based on the value of the sequence identity (calculated using gaps as non-indentical residues) after separately aligning each of the obtained sequences with each of the Arabidopsis sequences, using Muscle [ 77].
Based on the new correspondence result, we can construct new dynamic reference by linear combination, which will get more accurate aligning result.
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