The specificity of these primer pairs were initially evaluated in silico using sequences obtained from GenBank and aligned with ClustalX (Table S1).
This "Virtobot System" allows for automated post-mortem biopsies and is composed of a six-axis industrial robotic arm (Stäubli TX90L; Stäubli, Freienbach, Switzerland) that has been mounted to an external axis, aligned with the CT table [ 28].
The Seg-3 sequences generated (Table 1), were aligned with other published sequences (Table 2) using ClustalX version 1.8 [49].
The subject was initially seated, with both hands placed on the table and the glass placed at 400 mm from the edge of the table aligned with the subject sagittal plane.
The students reported that they acquired insight into their own profession, a theme that aligned with the course objectives (Table 1).
Students valued the growth that came from being challenged in the course; this theme aligned with the course objectives (Table 1).
In total 129 EF-hands were aligned with one another (Supplementary Table 1).
The levels of perceived competence were generally aligned with the physicians' specialty (Table 4).
Molecular phylogenetic trees were inferred using regions of selected vertebrate Pax amino acid sequences that were unambiguously aligned with no gaps (supplementary table S5, Supplementary Material online).
The ssrA genes of 10 GBS strains which represent 7 serotypes including the 5 most commonly occurring serotypes (Ia, Ib, II, III, V) were sequenced (Sequiserve, Vaterstetten, Germany) and aligned with other related streptococci (Table 2) ssrA sequences generated in this study or available on the tmRNA website [ 22].
All the genes sequenced in the present study except GH7 from R. miehei that was too short to produce any significant alignment and the genes found in NCBI (see Table 3) were aligned with MUSCLE before curating the alignments with Gblocks and building of phylogenetic trees with PhyML (Dereeper et al. 2008).
