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Contigs were aligned with alignment parameters set to > 97% identity and > 40 nucleotides overlap using Sequencher (Gene Codes Corp., Ann Arbor, MI, USA).
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Smad and receptor nucleotide sequences were aligned with protein alignment as guide using RevTrans-1.4.
Amino acid sequences were aligned with MAFFT alignment software (Katoh and Toh 2010) using default parameters and visualized and edited in Geneious v7.1.7.
Protein sequences in each cluster were aligned with mafft_wrapper.py, each alignment was trimmed with pep_gblocks_wrapper.py, and all alignments were finally concatenated into a supermatrix.
This error is propagated in the next step, where the suffix of the blue alignment is aligned with the prefix of the red alignment.
All sequences were aligned with default BLAST alignment parameters to the UCD10X v1.0 pseudomolecules to identify the PUN1 sequence.
All plant genes were aligned with multiple sequence alignment using the CLUSTAW program http://align.genome.jp/.jp/
All data sets were aligned with the RNA SALSA alignment software [ 30], considering rRNA secondary structures.
The collected amino acid sequences of PARP catalytic domains were aligned with MUSCLE3.8.31 multiple alignment tool, using default settings [53].
Sequences were aligned with MAFFT (multiple alignment using fast Fourier transformation, v6.832b) using the "G-INS-I" parameters [ 28].
Orthologs were defined as proteins that were reciprocal best matches when all proteins from both genomes were aligned, with a minimum alignment of 60% of both proteins.
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