Exact(60)
Regions of the optimized alignment which could not be reliably aligned were eliminated from analysis (data alignment available upon request), resulting in an alignment of 525 bp for 16S, 520 bp for 12S and 1,149 bp for the Cytb dataset, for a total of 2,194 bp.
Next, sequences that were not aligned were subjected to translated sequence alignments to the same viral references, which were translated in six frames.
After alignment, ambiguous regions (i.e. containing gaps and/or poorly aligned) were removed with Gblocks (v0.91b).
The multiple alignments were manually inspected and edited, and only positions unambiguously aligned were included in the final analysis.
After alignment, ambiguous regions (i.e. containing gaps and/or poorly aligned) were removed with Gblocks (v0.91b) [ 66].
Because Sanger SNPs were identified by alignment to the reference sequence, regions that could not be aligned were inaccessible to SNP discovery and thus not comparable with array genotypes.
After alignment, ambiguous regions (i.e. containing gaps and/or poorly aligned) were removed with GBLOCKS (v0.91b) using the following parameters: minimum length of a block after gap cleaning of 10, no gap positions were allowed in the final alignment, all segments with contiguous non-conserved positions bigger than 8 were rejected and minimum number of sequences for a flank position: 85%.
After alignment, ambiguous regions (i.e., containing gaps and/or being poorly aligned) were removed with Gblocks v0.91b (Castresana 2000) using the following parameters: The minimal length of a block after gap clearing was set to 10 and no gap positions in the final alignment were allowed.
After alignment, ambiguous regions (i.e. containing gaps and/or are poorly aligned) were removed with Gblocks (v0.91b, [ 39]) using the following parameters: minimum length of a block after gap cleaning = 10; no gap positions were allowed in the final alignment; all segments with contiguous nonconserved positions bigger than 8 were rejected; minimum number of sequences for a flank position = 85%.
At each step, the parts outside the central GTPase domain, which often showed no homology across subfamilies (and therefore should not be aligned), were trimmed to facilitate the production of the next multiple sequence alignment.
In the present work, tissue patches containing human microvessels that were either randomly oriented or aligned were placed acutely on rat hearts post-infarction and for each case it was determined whether rapid inosculation could occur and perfusion of the patch could be maintained for 6 days in an infarct environment.
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