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The sequences of each group were aligned to obtain sequence logo of the translation initiation site using WebLogo 3, version 2.8.2 (http://weblogo.berkeley.edu/).edu/
Annotations showing independent kDNA integrations in LINE-1 were analyzed, and representative sequences were aligned to obtain probes targeting the hotspots of DNA integration (Figure S1).
For this, 10 or more trials were aligned to obtain mean responses for each neuron.
Matching contigs were aligned to obtain consensus sequences for the coding region of interest.
These 2 contigs were aligned to obtain the sequence of the entire 16S rRNA gene (1.5 kb).
Replicate sequences from each sample were aligned to obtain a consensus nucleotide sequence, and these consensus sequences were used in the final alignment (Fig. 4).
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Samples were abraded using SiC paper up to 120, 180, 600 and 1200 in a randomly or aligned manner to obtain different topographies and roughness levels in the range of 0.026 to 1.324 μm.
The obtained consensus structure is then matched with every aligned sequence to obtain separate parse trees.
The coverage for each base from the first to last nt in an annotated CDS was summed then divided by 32 (i.e., the length of each aligned read) to obtain the RNA-Seq coverage for that gene before normalization.
The sequences in the latter group were assembled into subgroups representing established substrate specificities (TyrAa, TyrAp and TyrAc) and were aligned separately to obtain overall consensus sequences for cyclohexadienyl-substrate core segments.
Finally, 2195481 and 1967523 clean tags were obtained and mapped to genome among which 1914805 and 2344576 clean tags were aligned to a gene obtaining 1849271 and 2310867 unique tags for control and ABA treated libraries, respectively.
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CEO of Professional Science Editing for Scientists @ prosciediting.com