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This tool assembled the BAM (binary alignment map) and BAI (binary alignment index) files and aligned them to the rCRS using TMAP Smith Waterman alignment algorithm [22].
To address this question, we obtained all of the original DGRP sequencing reads and aligned them to the iso-1 reference alignment (File S1).
To evaluate the quality of the assembled transcripts, we aligned them to the reference genome with the spliced alignment program ESTmapper/sim4db [ 19], retaining only the best match for each transcript.
To "correct" for mutations, the researchers took dozens of known HERV-K HML2) HERV-K HML2nd aligned them to create a sequences "consensus" sequence.
Shape screening generated different conformers for the rest of drugs and aligned them to each template to identify common pharmacophoric features between each pair of drugs.
We took RP288 and orthologous proteins from the additional 12 Rickettsia genomes and aligned them to 70 VirB7 and VirB7-like proteins retrieved from GenBank, a subset of which reveals the limited sequence conservation in this protein family (Figure 8).
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We concatenated sequences only after the alignment instead of before aligning them to avoid falsely aligning sequences from different domains.
We predicted the boundaries of rRNA genes by aligning them to rRNA sequences from the published cnidarians and checking the alignments manually.
After aligning reads from the parents to these contigs, we align them to the reference genome to determine whether they have a better alignment to another location.
Such geospatially connecting video sequences can be used to join separate 3D models by aligning them to the common camera trajectories.
Nevertheless, it was not possible to precisely resolve the number of repeats composing these elements, neither by aligning them to a reference sequence, nor by de novo assembly.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com