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The largest aligned segment contains the etpCDEFGHIJKLMNO gene cluster encoding a functional T2SS in pO157.
The impact of systematic errors on the estimation of covariation was tested in an alignment of triosephosphate isomerase by randomly shifting a fraction of the sequences in one aligned segment left or right by 1 residue.
As before, ERK and CvP-bias were calculated for each aligned segment.
For each BLAST hit (aligned segment), we extracted the flanking genome sequence up to several thousand nucleotides 5' and 3' of the hit.
Completeness, also known as integrity or transcriptome coverage, is the ratio of the sum of all unique aligned segment length to the reference length.
Remarkably, each of these regions corresponds to an aligned segment in the multiple sequence analysis, although the spacing between individual putative β-strands varies between the major phylogenetic groups (Fig. 4).
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Gblocks [ 22] was used to remove poorly aligned segments of alignments, and the minimum length of a block was set to 100, and the maximum number of consecutive non-conserved positions to four.
In practice, dissectHMMER considers short non-globular aligned segments of ≤10 alignment positions as part of the fold-critical segments.
More strikingly, we noted a strong clustering of variants in smaller regions where we had extended sequence from the ends of aligned segments during the iterative alignment process and where we had replaced sequence with the JR-assembled contigs.
Multiple DNA sequence alignments were inspected and any ambiguously aligned segments were removed prior to phylogenetic analysis.
Then, where nc(M1) is the non-coding region of M1 and R' is one m-kb long region in nc(M1), and S R, R') is the sum of local alignment scores of all pairs of aligned segments in R and R' output from CHAOS [ 16].
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