Sentence examples for aligned reliably from inspiring English sources
The remainder of the sequence segments could not be aligned reliably based only on the sequences and structures present in the alignment.
However, these criteria may excessively filter out regions with insertion/deletion events that may still be aligned reliably, and these regions might be desirable for other purposes such as detection of positive selection.
Regions that could not be aligned reliably were excluded from subsequent phylogenetic analysis.
11) Are the phylogenetic trees based on the entire aligned sequence, or on specific sequence blocks (domains) that could be aligned reliably?
Some archaeal ModA proteins have been characterized; these sequences were excluded from the tree because they could not be aligned reliably with the bacterial sequences.
Thus, we have developed a scanning approach that will find sites in mixed data that can include one or more clades of sequences (each of which can be aligned reliably) as well as sequences which cannot be aligned reliably to any other sequences.
Regions with high nucleotide diversity were difficult to align reliably.
Such approaches may be more difficult in plants, as plant non-coding regions can be difficult to align reliably due to their dynamic nature and often high content of repetitive elements.
A dependence on a single alignment may be particularly harmful when searching for regulatory motifs, such as transcription factor binding sites (TFBS), which are difficult to align reliably due to their short lengths (6-15 nucleotides) and tolerance of degenerate nucleotides [ 7].
A total of 456 complete amino acid sequences (452 available in databases and 4 newly predicted) can be reliably aligned, and the alignment of representative sequences from each of the 5 subfamilies is shown in Figure 1.
Regions of the optimized alignment which could not be reliably aligned were eliminated from analysis (data alignment available upon request), resulting in an alignment of 525 bp for 16S, 520 bp for 12S and 1,149 bp for the Cytb dataset, for a total of 2,194 bp.
