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Each aligned location was extended downstream to a length of 150 bp.
We filtered a small number of sites for which the manifest file listed SNP alleles that differed from those in dbSNP at the aligned location.
Similar(58)
If both alignment methods were able to align a molecule, and their aligned locations were similar (within half the length of the molecule), a consensus was reached, and we included the OMBlast result in our integrated list.
The resulting alignment file (in SAM format), consisting of the read sequences and aligned locations if available, was then passed to Velvet along with the reference ESTs.
Close inspections of aligned locations scored as absences were often suggestive of degenerate residues of blocks.
Finally, if both methods were able to align a molecule, but the aligned locations were different (more than half the length of the molecule apart), we would exclude both alignments.
In each pairwise combination, only a small number of S. pombe G4 motifs (20 to 31) overlapped a G4 motif in the aligned homologous location in another species (Table 1).
All HT-based alignment tools use the same seed-and-extend strategy [ 11, 12], which proceeds by searching for candidate alignment regions (CARs), aligning each location, and reporting the best alignments.
According to the standard criteria for novel miRNA identification, extended sequences at the aligned genome locations have a propensity to form hairpin structures.
If a transcript were aligned multiple locations with more than 80%% matches, or a probe hit multiple transcripts from different annotation projects mapped on different locations, these hit probes were eliminated.
It would then follow that there should be no gain from aligning the location of the arrow and the location of the to-be-updated object, contrary to the results of Experiments 2a – 2a.
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