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Just like in chess, individual fighting moves are useless on their own; performed in a calculated sequence, however, they can take an opponent out.
Superposition of the domains reveals a strong degree of structural similarity with a backbone RMSD of ~3.2 Å (Supplementary Fig. 2a) and a calculated sequence similarity for the structurally aligned regions of 29% (identity 19%), suggesting a common evolutionary origin.
The nucleotide sequence for diverse blaCTX-M genes was aligned in a search for common sequences.
Briefly, sequences were manually aligned in a secondary structure-based database using the program RNA (19).
Thus, the sequences flanking all edited or unedited cytidines were aligned in a matrix.
Nucleotide sequences of pncA genes found within mycobacteria where aligned in an effort to ascertain the significance of any variability in sequence.
In total ∼83, 000 aa were aligned in 73 sequences.
was aligned in order to obtain a consensus sequence.
To compensate for pyrosequencing errors in homo polymeric regions, the generated consensus sequences were aligned against a reference sequence and deletions in the sequencing data were corrected.
Both sequences were aligned, and a final sequence was created.
The sequences were aligned in MEGA 7 70.
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