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The aligned coverage of the human genome for GM12878 and HCT116 was 5.5× and 4.6×, respectively, while the Ray et al. data gave 37× and 25× coverage.
Copy number was inferred from aligned coverage of Rmaps, prior to assembly, in a manner analogous to read-depth based methods for detecting copy number variants from second generation sequencing data (methods).
We similarly compared OPTIMA and Gentig on the two human cell line results, shown in Tables 3 and 4, in order to calculate how much mapping data would be needed for sufficient aligned coverage of the human genome to enable structural variation analysis.
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These were then filtered to extract only those deletions with high, precisely aligned coverage on both sides of the deletion.
Summed aligned coverage indicates the sum of the aligned region of contigs across the 13 mitochondrial genes.
Ten of these cases were sequenced to ∼30× aligned coverage in tumour and normal using whole genome shotgun sequencing.
First, callable reference regions are identified, with sufficiently aligned coverage.
We used a percent identity of 95% and alignment coverage of 0.5, the ratio of aligned length to total FL-cDNA length, as filtration criteria.
Here, we describe a novel algorithm (Iterative Correction of Reference Nucleotides) that iteratively aligns deep coverage of short sequencing reads to correct errors in reference genome sequences and evaluate their accuracy.
Homologous proteins were identified by BLASTP on the criteria of an E-value cutoff of 1e-5, and minimum aligned sequence length coverage of 50% of a query sequence.
Homologous proteins were identified by the BLAST Basic Local Alignment Search Tooll) program [ 83] with an E value cutoff of 1e-5 and a minimum aligned sequence length coverage of 50%% of a query sequence.
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