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"aligned against" is a correct and usable phrase in written English.
It is often used to describe a group or individual that is in opposition to another group or individual. Example: The two political parties are aligned against each other on the issue of healthcare reform.
Exact(60)
The forces aligned against this are powerful.
Jews and Muslims often found themselves aligned against Christians.
No single wall separates East and West; no one rival superpower is aligned against us.
With such powerful forces aligned against it, the theme park for Sighisoara died.
But on Thursday, many city and state lawmakers were aligned against Mr. Sprayregen.
And usually, the six council members are aligned against Ms. Barcher, also a Democrat.
"We may very well find that we are aligned against a common enemy.
Remaining sequences were aligned against the SILVA SSU eukaryotic aligned database.
But Mr. Moore found himself aligned against Democrats and Republicans alike.
Single contigs were aligned against the parental synV sequence and visualised using the Integrative Genome Viewer26.
Then, homologous genome sequences were aligned against the matching proteins using Genewise to define gene models.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com