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Matches to rodent and primate species were discounted, as were matches where the initial AUG codon did not align with the reference sequence.
Additionally, their position was confirmed by identification and alignment of reads containing both the sequence of the reference genome and those in the contig that did not align with the reference.
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The sequence reads from parent strains 16D and ER18 were aligned with the reference sequence of strain S288C.
For criterion (2), strand refers to whether a read aligned with the reference genome (plus) or as the reverse complement (minus).
The ENAM gene sequences from the patient were aligned with the reference sequence (GenBank accession no. AY167999) using VectorNTI software.
In this network each local ontology network is aligned with the reference ontology network so that search queries, CVs, and job offers can be mediated through these alignments from any ES.
The sequence data of the 3000 genomes were aligned with the reference genome Nipponbare.
Moreover, approximately 4.77 G high-quality raw databases were aligned with the reference sequence of cultivar 9311 using the SOAPaligner (Li et al. [2008]).
Then, contigs were aligned with the reference genome to construct the draft chloroplast genome of each species in Geneious 9.0.5 software (http://www.geneious.com).
The current calculated at (Drain) and (Source) contact are quite aligned with the reference tool while the (Bulk) component is lower due to the different generation value of II terms (not shown here).
Paired images were visually aligned with the reference to an outline of the skull and cropped to the same size by customized software (Image Rugle 2009 Medic Engineering Inc., Kyoto, Japan).
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