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Examination of the TFBS SNPs in the RCE promoter regions (supplementary table S3, Supplementary Material online) reveals several cases where our predictions align with additional biological information to generate hypotheses that can be tested experimentally.
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sp cox1 sequences from our assembly and sequencing were manually aligned with additional sequences from other Hydra and outgroup species available from GenBank in the SeaView editor [ 23].
The PCR product (637 bp) was sequenced and aligned with additional sequences from the Cronobacter PubMLST database in MEGA (Molecular Evolutionary Genetics Analysis) software version 5.2 [ 19] using the ClustalW algorithm.
The Filter Tank can be positioned as needed to avoid an obstacle, keep it out of the lawn, or be best aligned with additional water storage tanks.
Due to the extensive divergence of their sequences [ 11], human PRNP did not align with PrP2s (Additional data file 3).
Further modifications to stretched microthreads, such as carbodiimide crosslinking, could generate microthreads to direct cell orientation and align tissue deposition, with additional resistance to degradation for use as a long-term scaffold for tissue regeneration.
The variable regions were aligned with two additional X. fastidiosa genomes, M12 [21], which is a fully annotated X. fastidiosa subsp. multiplex genome (GenBank CP000941.1), and Ann1 [32], which is a draft X. fastidiosa subsp. sandyi genome (GenBank AAAM00000000.3).
Sequences were aligned with 76 additional full-length WNV sequences available in GenBank using Clustal Omega 18 and edited manually.
The corresponding amino acid sequences of each haplotype were predicted by BlastX and aligned with ClustalW (Additional file 5).
The intention was that evaluation committee would use the public comments aligned with the additional information to inform their final decision.
Many of these barriers aligned with ecoregions (Additional file 1), suggesting that barriers predicted by our model do indeed delineate areas that represent valid biogeographic barriers.
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CEO of Professional Science Editing for Scientists @ prosciediting.com