The topic guideline for interviews with former DTTBs was developed to broadly align with the sections of the self-administered questionnaire and made use of open questions so as to obtain further, clarifying and explanatory information.
Moreover, ChAT bands are useful to align the different sections on different confocal planes ensuring the dendrites are reconstructed properly.
Models from serial sections often have high quality images in the plane of section, however, difficulties in aligning the serial sections can lead to poor quality images when viewed in other planes.
I think it was a great idea to split up our research efforts into three weeks the way we did, because it had aligned with the section-wide lectures with Kip.
The results are then sent to the operation 'prettyplot' (from EBI SOAPLab) to generate an alignment plot, highlighting the aligned sections for the group of ESTs.
First, we visually compared alignments for congruence in their ability to align sections of the alignments (especially the inner core) using AltAVisT [54] and the overall sequence identity calculated from each alignment.
If you have more than six pieces of paper, you may want to punch holes in each book section separately, but be sure to measure where the holes go so that they will align neatly when you combine the sections.
We then outlined the TLCd (as well as fiduciary marks used to align the drawings of consecutive sections) using a 20× (N.A. = 0.50) objective lens.
In addition, the evaporation of polyvinyl alcohol, which acted as a dispersing agent, from the cross-section helps align the GOs vertically in the cross-section.
Care was taken to ensure that all the landmarks on the sections were aligned with the map.
The sections were aligned with Neurolucida and visualized with NeuroExplorer, rendering visible the surface of the midbrain and the nuclei of interest.
