Exact(1)
In order to align overlapping mate reads and produce accurate composite fragments, we developed the SHERA software pacakge, a collection of computer code written in the PERL programming language.
Similar(59)
In the family Brassicaceae, model species Arabidopsis thaliana was sequenced by the Sanger method using aligned overlapping BACs [ 4].
A major application in genome sequencing is de novo assembly, which aligns overlapping reads into super-sequences known as contigs and uses paired-end (PE) reads to further connect contigs into scaffolds [ 1].
Significantly aligned, overlapping sub-sequences were merged and regions in other species that mapped to the same sub-sequence in N. vitripennis were identified to yield one inclusive dataset (see Methods).
Initially, the Newbler developers said that these contigs are not parts of actual transcripts and are therefore not reported, but we traced a few of these fragments and found that they were made up of well aligned, overlapping reads that had been split into contigs and then discarded.
Numerical programming-trapping iterations were employed to examine the distribution of electron injections and trapped charges in the charge-coupled cells, and to discuss the differences of physical mechanisms among the aligned, overlapped, and underlapped cells.
Without correction, codons and proteins will align in overlapping, frame-shifted segments, and to our knowledge no pyrosequencing study has previously addressed this issue.
Each of the aligned overlapping nonamer (9-mer) positions represent a possible antigenic core binding domain for human leukocyte antigen (HLA; human MHC) molecules and T-cell receptors [ 16, 17]; this assumption is based on the fact that there are large array of HLAs with different binding specificities in the human population [ 17].
Wolfson said that Buckel became interested in and committed to the fight for transgender rights long before the cause received widespread attention — long before even most gay-rights advocates acknowledged that the gay and lesbian and the transgender causes were "aligned and overlapping".
WASHINGTON — As it devises a new Afghanistan policy, the Obama administration confronts a complex geopolitical puzzle: two embattled governments, in Afghanistan and Pakistan; numerous militias aligned with overlapping Islamist factions; and hidden in the factions' midst, the foe that brought the United States to the region eight years ago, Al Qaeda.
We do not believe that the sequences selected were due to PCR over-amplification because most sequences aligning in overlapping positions varied in their terminal nucleotide position (not shown).
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