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Sequences for all individuals and the haplotype alignment are deposited as alignment ALIGN_001234 in the EMBL-Align database that can be accessed by the EBI sequence retrieval system [ 63].
After alignment and trimming, the resulting aligned database used for UPGMA and neighbor-joining analyses comprised only the ITS-2 region.
We also show the alignment error of the initial (not) aligned database (None).
Here, we introduce an aligned database of protein kinase structures that can be efficiently explored by sequence, structure, or by ATP pocket ligand (type or similarity).
This alignment forms the basis for all the phylogenetic analysis regarding the XIP group of MIPs and is available as ALIGN_001171 in the EMBL-align database.
Therefore, we first investigated the size of confidence sets for topologies obtained with real data: eight-taxon nucleotide data sets from the EMBL-ALIGN database.
We iteratively constructed eight-taxon subsets of each data set in the EMBL-ALIGN database until we found a subset for which the GLS statistic could be calculated.
Gaps and positions of doubtful homology in the multiple alignments were removed using Gblocks [ 22]; only alignments longer than 1000 nucleotides were kept: 108 out of 539 in the EMBL-ALIGN database.
The alignments were manually inspected and adjusted as mentioned above and used for phylogenetic analysis of PpHIP1 1 and the PpXIPs and are available in the EMBL-align database as ALIGN_001169 respectively ALIGN_001170.
The alignment of sequences of each subset and that of full consensus sequences related to this work have been deposited in the EMBL-Align Database and can be accessed through this database under # ALIGN_000563 and no.
Indeed, both in simulations (not shown) and for biological sequences obtained from the EMBL-ALIGN database we observed that in many cases the calculation of the GLS statistic was not possible due to the singularity of the distance matrix.
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