The phrase "align back" is not standard in written English and may cause confusion.
It could be used in contexts where you want to indicate returning to a previous alignment or position, but it is not commonly recognized.
Example: "After the meeting, we need to align back on our project goals to ensure everyone is on the same page."
Alternatives: "realign" or "return to alignment."
they've taken the time to see what customers are interested in and are building out partnerships that align back to customer's install base and business requirements," Wang explained.
In our case, 9% of the reads did not align back to the transcript reference sequences.
All PE reads were used to align back to these assembled sequences using the Bowtie2 (v2.0.0-beta5 v2.0.0-beta54].
Many reads were impossible to align back to the contigs, possibly because the redundant contigs had been removed in the previous analysis (Step 3 in Figure 1, with the longest contigs per locus selected by Oases).
None of the original reads from the other species align back to the predicted VRN2 transcript, confiming that the VRN2 transcript is not present in the RNA-seq data sets of non-perennial species.
The idea behind this strategy is that the ends of the reads, which align back to the reference genome, map back to specific positions of an expected distance according to information from stored DNA libraries.
For each species, the 80 protein-coding gene sequences were translated to amino acids, were aligned using MAFFT aligner tool [ 43] and finally, the respective nucleotide sequences were aligned back based on the translated amino acids' alignment using back-translator tool from TreeBeST (http://treesoft.sourceforge.net/treebest.shtml).shtml
We define a true alignment as a situation when a read is aligned back into the same position from which it was generated.
Without using alignment thresholds, more than 99.9% of all sequences could be aligned back to the reference.
To assay the assembly quality, all the PE reads were aligned back to these contigs by using Bowtie2 program (v2.0.0-beta5 v2.0.0-beta5 57gned rand was calculathe.
More than 98% of the Illumina reads from the two PCR-free libraries can be aligned back to the final assembly.
