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Legend: biological GS: biological gold standard; CSH: cross-species hybridization; Align: alignment; mis: mismatch; U: union.
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This section illustrates the MP-Align alignment algorithm.
For each of the 3 matches, the number of ambiguous bases (International Union of Pure and Applied Chemistry codes) and the percent aligned (alignment length as percentage of query length) were calculated.
In addition to the identified orthologs, 30-40% of the remaining translated CDS aligned (alignment length > 50 amino acids) with significant sequence similarity (E-value < 1 × 10−20) to protein coding sequences of the other vertebrates (Table 1).
Amino acid sequences were aligned (alignments available on request to DJM) and analysed by Bayesian and ML methods.
After aligning, alignments in each query were sorted according to the score and then the identity.
It is known, however, that when unrelated amino acid sequences with unusual composition are aligned, alignments with high score occur with implausibly high frequency.
The two channels were manually aligned, via alignment grid, to within 1 2 pixels.
These features include aligned columns, alignment replicates, iterations without improvement, insertion, deletion, shuffling, and examining forward and reverse strands.
Contigs were aligned with alignment parameters set to > 97% identity and > 40 nucleotides overlap using Sequencher (Gene Codes Corp., Ann Arbor, MI, USA).
Color channels were carefully aligned using alignment parameter from control measurements with 0.5 µm diameter multi-spectral fluorescent beads (Life technologies).
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