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In a) the '+' indicates the location where the algal samples were collected.
Brown algal samples were treated with 2% CaCl2 at 80 °C, 0.2 M HCl, 3%Na2CO33, 50 mM CDTA and 4 M NaOH with 1% v/v NaBH4.
All algal samples were refrigerated up to 24 h prior to processing.
Algal samples were analyzed for chlorophyll-a concentration using USGS methods (Yin 2005).
Algal samples were irradiated at 55 μmol photons m−2 s−1.
30 Marine algal samples were collected from the different locations around the coast of Karachi, Pakistan and about 20 fresh water algal samples were collected from fresh water ponds and lake.
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The wet weight of sample of collected algal samples was taken and shade dried.
Algal samples are characterized depending on the conventions for that particular lineage, usually including microscopic and molecular characterization, and sometimes culturing studies.
After 72 h of treatment, 1 mL of algal samples was exposed during 15 min to 0.2 mM of FDA in the dark.
After 72 h of treatment, 1 mL of algal samples was exposed during 15 min to 0.2 mM of H2DCFDA in the dark.
Isolation of DNA from the majority of algal samples was thus performed according to a protocol similar to that for preparation of high-molecular-weight samples in agarose plugs (Sykorova et al. 2006).
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