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A volume of 100 mL algal cells was centrifuged at 5000 ×g for 10 min and the algal pellets were washed off with distilled water.
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For 250 mL experiments, the fungal pellets were produced in PDB or 1% TWS for 3 days, excess liquid was removed using a Pasteur pipette and the pellets were washed with sterile water followed by algal medium.
Before mixing with microalgal cultures, pellets were washed twice in sterile water, followed by sterile algal media.
For algal biomass analysis, a 10 mL sample volume was centrifuged at 8000 rpm for 10 min, and the centrifuged pellets were washed twice with distilled water to remove the salts and solids.
Cell pellets were washed with water.
Pellets were washed thrice with distilled water.
The cell pellets were washed twice with distilled water.
The supernatant was discarded and pellets were washed with PBS.
Pellets were washed three times with TS buffer.
RNA pellets were washed with 75% ethanol and dried.
Cell pellets were washed gently with saline (2 ml).
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