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Algal inoculation, or so-called algalization, has been applied as an alternative to fertilizer because it exerts a positive effect on soil properties (Aiyer et al., 1972) and provides better N-availability than chemical fertilizers (Tirol et al., 1983).
Cultures were carried out in 250 mL flasks containing 150 mL media and a 10% (v/v) algal inoculation.
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Without inoculation, algal biomass still produced H2 due to the activities of satellite bacteria associated with algal cultures.
The cell density was estimated at every 24 h intervals; the total nitrogen and phosphorous were analysed in culture medium, before inoculation of algal cells and after 7 days of culture.
At inoculation, the algal cultures were supplied with copper or selenium nanoparticles (average size about 100 nm) carboxylated with citric acid (nCu-Citr or nSe-Citr, respectively) [6, 7], obtained from the Ukrainian State Scientific Research Institute Resourcee' (Kyiv, Ukraine), to final concentrations ranging from 0.67 to 40 mg L−1 (nCu-Citr) and from 0.07 to 4 mg L−1 (nSe-Citr).
After several re-inoculation steps, algal cultures were grown axenically in liquid Woods Hole MBL medium.
Introduction of algae into the 250-mL Erlenmeyer flasks occurred at 0 h by inoculation of 0.1 mL algal biomass (107 algal cells/mL) into 100 mL test solutions.
After inoculation of wastewater with algal paste, the following algal concentrations: 0, 3.2, 13.9, and 20.0 mg L−1 chlorophyll-a were obtained for both LSW and MSW.
Homogenous algal suspension (5 ml) was taken for inoculation.
The algal biomass at late exponential phase (5~7 days after inoculation, 2~3 g L−1) was used to inoculate the experimental systems.
Specific inoculations may become successful or may not compete the contaminating algal species.
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