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The 18S rDNA DGGE system resolved eight bands from algal DNA, but many of the sequences separated were not fungal, whereas the 28S rDNA system resolved seven bands that were all identified as ascomycetes.
A phylogenetic tree was made by comparing a conserved part of this gene with homologous sequences from other nuclear cytoplasmic large DNA viruses (NCLDV, [45]), with emphasis on algal DNA viruses (Figure 7).
Total algal DNA was isolated from 1 g wet filaments.
Surprisingly, all the clones were positive, indicating that they also have algal DNA.
To distinguish clones containing EsV-1 DNA linked to algal DNA from clones containing only EsV-1 DNA, the isolated positive clones were screened with labelled algal DNA from Vic88 12 15 f female gametophytes.
This therefore supports the contention that region A is composed of viral DNA framed by algal DNA.
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Visualizing algal spore DNA under a light microscope can be difficult due to the impermeability of the highly resistant spore wall to dyes and stains used in normal staining procedures.
Our study shows that the capture (and fixation) of algal host DNA has been rare in the evolutionary history of CVs and cannot explain the vast majority of non-ancestral CV genes.
We screened samples of algal genomic DNA by Southern hybridization using radioactively labeled oligonucleotides as probes (fig. 4) to unveil a possible occurrence of other telomere-like minisatellites in the respective genomes and to possibly identify candidate telomeric sequences in samples with no detected telomerase activity.
Based on its relatively high percentage of coding regions (42 %), large gene repertoire (64 genes) and limited extent of repeats (5.6 %), the L. incisa mitochondrial genome can be classified as belonging to the "ancestral" type of algal mitochondrial DNA, as opposed to the "reduced-derived" pattern observed in Pedinomonas minor and Chlorophyceae [ 10].
Similar searches against green algal genomic DNAs also found some homologous regions to the land plant CslB/H/E/G/J genes.
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