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Bumblebees, B. terrestris, were purchased from Koppert (Berkel en Rodenrijs ,Netherlands) and kept in an air-controlled room at constant 60% humidity and 25°C temperature and a 12/12 hours day/night regime.
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The animals were housed under pathogen-free conditions in specifically designed air-controlled rooms with a 12-h light/dark cycle and fed with food and sterile water ad libitum.
Briefly, the system is an air-tight, temperature-controlled room in which rates of oxygen consumption (VO2) and carbon dioxide production (VCO2) are calculated as the difference between the composition of entering and exiting air.
During these experiments, subjects breathed ambient air in a climate-controlled room; therefore, Cove and Pinsky's claim that an increase of CO is due to an increase in inhalation is unlikely.
However, the RDTs were shipped to the study sites by cold-chain transport, and the study site coordinator and the independent monitor assured that batches were used within the expiration date, as well as stored in air-conditioned, temperature-controlled rooms.
The cultures were bubbled with air and maintained in a temperature-controlled room at 25°C on a plexiglass shaking apparatus [ 28] under a photon irradiance of 150 μmol photons m-2 s-1 with continuous light provided by cool-light fluorescent lamps (Philips TLD30w/55).
After a 7-day period of adaptation, piglets (average body weight of 6.44 ± 0.39 kg) were housed individually in stainless steel metabolic cages (1.20 × 1.10 m) and maintained in an environmentally controlled room (25°C) by air conditioning, with electric light being provided between 8 00 AM and 8 00 PM [ 26].
The room was climate-controlled (room temperature 23 25°C, relative air humidity 30 58%) and protected against strong sunlight.
Mice were housed in duplex polycarbonate cages with a Shepherd Shack on ventilated racks providing 99.997% HEPA filtered air to each cage in a climate-controlled room under a standard 12 12 light dark cycle (lights on at 0600 h).
Place the drying racks in a temperature-controlled room or oven and assure a good air exchange.
The experiments were conducted in a temperature-controlled room under an aerated hood with humidified air to minimize evaporation.
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