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qPCR demonstrated dramatic induction of these mRNAs throughout the air pouch time course.
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Differential regulation of selected targets was validated by relative quantitative PCR in time course experiments with dissected air pouch membranes and murine peritoneal macrophages.
Infiltration of PMNs towards LIX (1-92) injected in a dorsal skin air pouch of Mmp8-/ mice was impaired at all time points compared to PMN infiltration in wild type mice, with an ∼2-fold lower number of PMNs seen at 8 and 12 h in knock out compared with wild type mice (Figure 5).
However, results of the time course analysis demonstrate that major transcriptional reprogramming in the air pouch membrane occurs several hours earlier.
Investigations were performed using carrageenan induced rat air pouch model.
Infiltration of the cells into the rat granuloma air pouch was also significantly inhibited by kaempferol.
To assess the anti-inflammatory effect of the extract, rat air pouch linings were examined histologically.
MX-68 (0.5 mg kg−1 week−1), as well as MTX, inhibited infiltration of leukocytes into the air pouch.
Inflammation was produced by adding carrageenan into a newly formed air pouch and prostaglandins produced.
In the rat air pouch model, oral dosing of CJ-13,610 and zileuton resulted in selective inhibition 5-LO Xactivity from pouch exudate and ex vivo rat whole blood with similar potency to in vitro assay.
They used subcutaneous air pouch rat model as well as knee joints of normal rabbits [70].
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