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Drying the mushrooms can also reduce the concentration of gyromitrin; ten days of open air desiccation leads to the loss of 90% of gyromitrin.
Controlled air desiccation and low temperature shock has been reported for increasing embryo germination frequency in B. napus[ 40, 48, 49].
The optimum frequency of embryo germination (86.5 ± 3.7%) was obtained by slow air desiccation of embryos followed by their culture at 18.0 ± 1°C in dark conditions till activation of radicle and plumule (Table 1).
The quantitative analysis of embryo germination (Table 1) showed that slow air desiccation resulted in more than three times higher (50.3 ± 19.8%) embryo germination percentage compared to direct culture of embryos (14.0 ± 4.7%), irrespective of the embryo induction temperature and conditions for incubation.
In our study, a combination of air desiccation followed by incubation of mature microspore embryos at 18°C in dark resulted in more than 90% germination frequency; this treatment for germination of microspore embryos has not been reported in B. napus doubled-haploid (DH) systems.
The embryos induced by the two temperatures (32°C and 18°C), from donor plants grown under low temperature conditions in growth chambers, were tested for their germination ability, under different desiccation treatments (slow air desiccation or direct culture without desiccation) and different temperature conditions (4°C for 10d, 18°C up to activation of radicle plumule, and 25°C continuously).
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During dry periods the sediment ponds may be exposed to air and desiccation effects (acidic sulfate salt formation).
Filters were archived at − 20°C in air-tight desiccation chambers until processed for oxidative screening and metal analysis.
The bacterial community on the anode of the batteries was tested for air tolerance and desiccation resistance over a period ranging from 2 days to 2 weeks.
These proteins have the unique ability to self assemble once secreted and they function at the fungal wall-air interface, limiting desiccation and providing protection against both chemical and enzymatic attack [ 53].
We report that the resulting levels of intracellular trehalose (∼80 mM) are able to confer increased resistance to the partial dehydration resulting from hypertonic stress, but do not enable survival of complete desiccation due to air drying.
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