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As panel designs mature it would be interesting to assess the effects of greater amplicon redundancy, for example, aiming for coverage of every target base with at least two amplicons (2x tiling) with different primer positions.
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There are several good summaries of raw data quality, but it is common to aim for coverage with high-quality bases to reach 20× or greater in 80 95% of the protein-coding sequences in each genome, after removal of ambiguously mapped reads and of duplicated reads (4, 55).
So far, it has laid fiber optic connections to about 30 percent of all the buildings; it is aiming for 60 percent coverage by the end of this year.
This week, at an international forum in Mexico City, country delegates from around the globe made clear that they are not only aiming for universal coverage but also rapidly getting there.
In the early years of Glonass's existence, a total of 43 satellites were put into orbit, with the authorities aiming for global coverage by 1991.
New formulations of pneumococcal conjugate vaccines with higher valency (i.e. 10 and 13 serotypes), aiming for higher coverage and wider use, will soon become available.
Aiming for optimal coverage of human populations, we suggest that 11 of these new WNV epitopes would be sufficient to cover from 48% to 93% of ethnic populations in various areas of the World.
Solexa Genome Analyzer IIx (Illumina, Little Chesterford, Essex, UK) was used to sequence each sample aiming for 1,000-fold 1,000-fold
It was introduced with a three-hour training session, repeated subsequently for those absent, aiming for complete coverage of admitting clinical staff.
Its spirit remains alive and kicking, with the world still aiming for universal coverage in terms of access, equity and quality of care (1).
'At this moment we are aiming for universal coverage in Bie and Malanje Provinces, with all partners committed to escalating enrolments throughout Angola.' There have been no more critical years for Angola than 2003.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com