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We proposed a high-performance implementation of a local tomography method aiming at removing the cupping effect.
A critical pre-processing procedure for detecting differentially expressed miRNAs is normalization, aiming at removing the between-array systematic bias.
The computational steps of the MRN method aiming at removing the bias due to the relative size of studied transcriptomes, are given below.
The MRN method follows the idea behind TMM and RLE normalization methods aiming at removing the bias due to the relative size of studied transcriptomes.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com