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A window of 100kb surrounding each AIM was analyzed, the average methylation status was calculated for the whole window and also for promoters, gene bodies and intergenic regions.
First, the main aim was analyzed comparing the treatment and wait list control group after the end of the first 8 weeks.
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After eliminating markers with more than 50% missing genotypes, less than 5% minor allele frequency (MAF), out of Hardy-Weinberg equilibrium in controls (p<0.0005), closely linked to others (<0.1 cM intermarker distance), 76 chromosome 12 AIMs and 88 genome-wide AIMs were analyzed (Tables S1 and S2).
For aim 2, ABPM was analyzed from an additional 45 subjects.
Each reference was analyzed aiming to identify all the mentioned concerns covered and solutions provided.
The relationship between the AIMs and methylation status was analyzed in AA, CA and HC populations.
Differences between post-intervention and post-control observations (aim 2) were analyzed using multilevel modeling (MLwiN 2.29).
To this aim, wine samples were analyzed with the whole set of sensors.
To address the last of these aims, the profiles were analyzed.
miR-204 downregulation bears potential of being analyzed aiming at this purpose.
To this aim, genes from the microarray are analyzed in an iterative process.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com