The phrase "aif expression" is not correct in written English.
It seems to be a typographical error or a misinterpretation of "AI expression."
Example: "The AI expression used in this context is quite innovative."
Alternatives: "AI phrase" or "artificial intelligence expression."
Recent work in Hq mutant mice, which have an 80% decrease in AIF expression, suggests that AIF depletion results in increased neuronal cell death [13].
To address whether AIF regulates intracellular PTEN oxidation, two pairs of shRNAs (shAIF#1 and shAIF#2) specifically against AIF were used to knockdown AIF expression, together with non-specific scramble shRNA (shNC) as a negative control.
Western blot analysis of AIF expression shows 76% downregulation of AIF protein expression in human islets exposed for 3 days to siRNA directed to AIF (siAIF) versus scrambled control (siScr, Fig. 3A).
As a multifunctional protein, we proposed that its pro-survival function in cancer cells with high AIF expression contributes to tumorigenesis, and its role as antagonist of PI3K signaling in cancer cells with low AIF expression during hypoxic environment is correlated with EMT and metastasis of cancers.
Ox-LDL-mediated upregulation of AIF expression was inhibited by actinomycin D, suggesting transcriptional regulation.
Furthermore, our immunocytochemistry results clearly showed that in untreated cells AIF was located in the cytoplasm and not the nucleus (figure 8G), whereas after addition of curcumin a time-dependent increase of AIF expression in the nucleus was observed, to reach maximum levels after 24 hours (figure Figure 8D F).
Two representative IHC images, respectively, for low and high AIF expressions of cancer tissues are shown in Fig EV5I.
Based on these findings, in this project we aim to explore the potential function of AIF-1 in female adipose tissue by mapping AIF-1 expression and possible release from cells within adipose tissue, as well as its relationship to obesity and metabolic disturbances.
AIF-1 expression preceded the expression of ED1, thus indicating a pre-phagocytic role.
A moderate 30% CR significantly reduced COX-2 and AIF-1 expression in the CT-2A astrocytoma.
We found that the anti-inflammatory effects of CR in the CT-2A astrocytoma were associated with (i) reduced nuclear NF-κB activation and subsequent DNA binding to promoters of proinflammatory genes, (ii) reduced COX-2 and AIF-1 expression, (iii) reduced CD68 and MIP-2 expression.
