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In order to investigate the intrinsic toxicity of different disease-associated expanded repeat RNA sequences, a set of constructs were generated in which a termination codon is inserted ahead of the repeat sequence such that the repeat tract is effectively shifted into the 3′UTR of the transcript in each case (Fig. 2F).
Once he arrived at the final album order, with nine months of recording still ahead of him, the sequence never changed.
The process allows the researchers to quickly engineer viruses to bind to a particular material, even if they don't know ahead of time what sequence of amino acids is likely to work.
It is important to obtain an accurate, representative sample of the genome ahead of full genome sequencing and annotation, and the end-sequencing of large numbers of BAC clones is an efficient strategy to achieve this goal.
A vehicle can send its status message when the vehicle ahead of it in the sequence finishes transmitting its status message.
The G0S2- EcoRI-5'F primer contains the Kozak sequence ahead of the start codon of G0S2 cDNA, whereas the G0S2- EcoRI-3'R primer contains the stop codon of G0S2 cDNA.
However, our study demonstrates that the template sequence ahead of the primer:template junction and the 3' end of the primer can have an effect on the amplification efficiency and should be considered in addition to Tm and GC measurements.
Insertion of the simulated data ahead of the FGB-int7 sequences should drive the alignment to conform more closely to the original mt tree.
We thank Martin Dohrmann for providing hexactinellid sequences ahead of publication and for useful comments on the manuscript and Catherine Vogler for commenting on the manuscript and for very productive discussions.
It was found that lawrencium coextracted with the trivalent ions, but the short half-life of the 256Lr isotope precluded a confirmation that it eluted ahead of Md3+ in the elution sequence.
A 13kb genomic fragment corresponding to the sequence located ahead of exon-1b was isolated and used to construct a series of luciferase reporter vectors following a 5'-deletion strategy based on the location of the predicted conserved regions.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com