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The results demonstrated that none of the GPs in our study had collaborative care agreements with secondary mental health professionals.
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The allosteric site (from residue 181 to 243) is straddling between the N-terminal and the compact globular core of the protein and shows an all-alpha structure composed by four alpha helices in agreement with secondary structure and disorder predictions (Figure 2b and 3).
Our findings suggest that the α-helix is longer than previously observed [ 19] and it is in agreement with secondary structure predictions (Table 2).
The α1 domain of the S. cerevisiae Matα1p also displayed these four alpha helices, in agreement with previous secondary structure prediction [14].
Its model was obtained by fold recognition strategy and presents an all-alpha fold composed from 4 alpha helices in agreement with the secondary structure predictions made by JPred [30] (Figure 3C) and with the structural class prediction made by PRECLASSPRO server [31].
We find it to adopt a partial α-helical conformation in agreement with bioinformatic secondary structure predictions.
This protection map is generally in good agreement with the secondary structure of β2m (shown in Figure 3 using line notations) deduced from the X-ray crystal structure of this protein, although some discrepancy does exist.
The aligned secondary structure of the template (Fig. 1a) is in good agreement with the predicted secondary structure of R843 (44% secondary structure match, with most of the secondary structure elements in the canonical fold predicted correctly).
In agreement with these findings, we also observed that patients with secondary T790M mutation showed significantly longer progression-free survival (p = 0.009).
The folding of RyR21 606·His6 was analyzed by circular dichroism spectroscopy resulting in α-helix and β-sheet content of ∼23% and ∼29%, respectively, at temperatures up to 35 °C, which is in agreement with sequence based secondary structure predictions.
With the significant threading score as well as a good agreement with the predicted secondary structure, the top model was selected for further analysis.
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