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After one hour of agitation on a rotary wheel at 4°C, the mixture was resuspended and the supernatant was collected.
The incubations were agitated either by inverting fifty times manually (∼3 min) or by continuous agitation on a rotating bench-top shaker (Biometra WT 17) at 50 rpm (rotations per minute).
Sure enough, as He tosses and turns with graceful agitation on a mattress in his Manhattan studio, strange things happen between the sheets.
In one terrific scene, the brokenhearted but feisty Inez (the scintillating Portia) turns her agitation on a passive, mousy white girl, Sonia (superbly played by Melissa Feldman), who sits almost unnoticed in a corner for most of the play.
Cultures were incubated in Erlenmeyer-flasks under constant agitation on a rotary shaker, maintained at 120 rpm.
These solutions were left in contact with 0.2 g of the adsorbent for 2 h with agitation on a shaker until the equilibrium was reached.
Similar(24)
Heparin and FGF-2 were combined (114 µg/200 ng) and incubated at room temperature (RT) for 15 min under mild agitation on an orbital shaker.
Plates were incubated at room temperature for 1.5 hr with agitation on an orbital shaker.
The assay plates were incubated for 2 h at room temperature or overnight with agitation on an orbital shaker.
Typically, 20 μL of 1X passive lysis buffer (reconstituted in water) was added to each well of the 96-well plate and subjected to brief agitation on an orbital shaker.
The flasks were purged for 10 min with N2, sealed, and then placed under agitation on an orbital shaker (Polyscience, USA) at 120 rpm and at room temperature for 50 days.
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