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The goal of this study was to evaluate an Agitated Soil Measurement (ASM) method for integrated on-the-go mapping of soil pH, soluble potassium and residual nitrate contents using ion-selective electrodes.
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Lunar soil was agitated at a frequency of 100 Hz using a vibration table with a containment chamber under a constant air flow.
A protocol was developed that employs a 1-g soil sample agitated overnight with 40 mL of reverse-osmosis de-ionized water for 20 h, and determination of 63 elements following three steps of centrifugation by inductively coupled plasma atomic emission spectrometry and inductively coupled plasma mass spectrometry the following day.
The soil pH was determined for a 1 2.5 soil:water mixture agitated for 1 h.
These were filled with slurries of a polluted soil and gently agitated for 5 days.
Exchangeable Ca, Mg, and Al were determined for a 1 10 soil:(1 mol L−1 KCl) mixture agitated for 10 min.
Electrical conductivity (EC) was determined following standard procedures proposed by Pauwels et al. (1992) where a 1 5 soil-solution ratio (10 g of < 2 mm soil and 50 ml distilled ionized water) was agitated for an hour and the readings from an EC meter, calibrated with 0.01 N KCl, recorded.
For the DOM collection, twenty grams of clean soil sample was placed in 200 ml deionized water and agitated on a reciprocal shaker at 200 rpm and 20 ± 1°C for 24 hours.
The soil was mixed with the aqueous sample of the plasmid, agitated overnight at room temperature and allowed to grow in rich medium supplemented with kanamycin.
Exchangeable P and K were determined for a 1 10 soil:Mehlich-1 mixture (0.05 mol L−1 HCl and 0.0125 mol L−1 H2SO4) agitated for 10 min.
The soil samples (1 g each) were each mixed with water (100 microliters) and pBAV1K-T5-gfp (10 micrograms) and agitated overnight at room temperature.
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