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e. Transfer slides to Coplin jar with 75% EtOH for 2 min; agitate periodically.
Transfer slides to first Coplin jar with 100% xylene for 1 min; agitate periodically.
Transfer slides to first Coplin jar with 100% EtOH for 2 min; agitate periodically.
Submerge slides in first Coplin jar of 100% EtOH for 1 min at room temperature; agitate periodically.
a. Submerge slides in first Coplin jar of 100% xylene for 5 min at room temperature; agitate periodically.
4. While Pretreat 2 solution is heating, dewax and dehydrate slides: a. Submerge slides in first Coplin jar of 100% xylene for 5 min at room temperature; agitate periodically.
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The cells were incubated on ice and agitated periodically for 10 minutes.
The vial was agitated periodically for 1 h to complete the ionic bonding of the doxorubicin.
Dried films (about 0.3 g) were weighed (initial dry weight) and immersed in beakers containing 50 ml distilled water at 23°C that were then sealed and periodically agitated for 24 hours.
The vial was periodically agitated to assist in the removal of the proteinaceous membrane (i.e. chorion).
Torres, who looked worn and agitated (he would get up periodically to smoke a cigarette outside), seemed paradoxically both grateful to and annoyed at Hoddenbach.
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