Sentence examples for aging assay from inspiring English sources
For the aging assay, degeneration was assessed by dye-filling [6].
One limitation due to the prolonged duration (7 days) of the aging assay is the production of progeny (200 300 per adult) by the aging animals.
Consistent with previous findings [14], the HDAC inhibitor trichostatin A (TSA) also suppressed neurodegeneration in the aging assay format (Figure S4).
Compounds were diluted into E. coli suspension or S-medium (starvation assay) to the desired concentrations. 1 ml of the final mixture was added per well of a 24-well polystyrene plate for the aging assay.
E. coli were grown overnight at 37°C in Luria broth (LB) media, pelleted by centrifugation, frozen at −70°C, and then resuspended at a final OD of 0.5 (595 nm) for the aging assay or an OD of 6.6 in nematode S-medium [29] (supplemented with 100× streptomycin/penicillin (Invitrogen, #15140-122) anystatintin (Sigma, #N1638)) for all other food based assays.
This strain derived from the wild-type strain CB15 ([ 17], ATCC 19089) by introduction of an in-frame deletion in the pilA gene (without this deletion, the aging assay in the microscopy flow chamber is not possible; presence of the adhesive pili prevents progeny cells from being removed from the flow chamber, so that they quickly accumulate).
C. elegans N2[wild-type] and GE24[pha-1 e2123)], E. coli food sources OP50 and HB101 (OP50 for compound efficacy and aging assays and HB101 for sensitized and food clearance assays) were obtained from the Caenorhabditis elegans Genetics Center.
This demonstrates that CLS, similar to aging assays in other organisms, is dependent on environmental conditions.
For aging assays, synchronous populations were obtained by allowing 5 10 hermaphrodites to lay eggs for 4 h to overnight.
Progenies used in aging assays and metabolite profiling experiments were prepared by mating wild-type animals of Canton-S background, which were backcrossed for seven generations.
Paraquat-induced oxidative stress assays were performed with fem-1 hc17) fem-1 hc17ites at 25 °C as for aging assays, except paraquat was added to NGM medium to 10 m m final concentration (Chermaphroditesst Chester, Pat USA).
