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A total of 21 C57BL/6 male mice were obtained from the National Institute on Aging aged rodent colonies: 5 (mature), 12 (old), and 24 (aged) month old ages (7 each) according to annotation standards for age classification from the Neuroscience Information Framework (NIF; http://neuinfo.org) [ 55].
Mice were obtained from Jackson Labs with the exception of the aged animals, which were obtained from the National Institute on Aging Aged Rodent Colony.
The F344xBN rats are long-lived with > 50% survival rate at 34 months of age (National Institute on Aging, Aged Rodent Colonies Handbook, www.nia.nih.gov/research/dab/aged-rodent-colonies-handbook/ strain-survival-information) and have significantly less pathological complications with normal aging as compared with the Fischer 344 (F344) rats (Bronson, 1990; Lipman et al., 1996).
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