To demonstrate the anti-aggregating effect of the magnesium-based anticoagulation, platelet aggregation studies were performed in blood samples from healthy donors collected by the Thromboexact™ (TE) and the standard hirudin-containing tube (S-Monovette™ Hirudin, Sarstedt, Nürmbrecht, Germany).
Aggregation studies indicate that non-peripherally substituted 1b is significantly less aggregated in aqueous media than peripherally substituted 1a.
Reagents for platelet aggregation studies were purchased from Wheecon Instruments Pvt . Ltd
Blood for platelet aggregation studies was withdrawn under medical supervision with prior consent from the volunteers.
Platelet aggregation studies were completed within 3 h of preparation of PRP.
All platelet aggregation studies were performed using a Chrono-log platelet aggregometer (Chrono-log Co., USA).
Discrete molecular dynamics (DMD) is a rapid sampling method used in protein folding and aggregation studies.
Larger particles were found to have an enhanced aggregation by both UV Vis turbidimetric and DLS aggregation studies.
In this work, we investigate the fibrillation of β-lactoglobulin, a common model protein for amyloid aggregation studies with relevant applications in the food industry.
Open image in new window Fig. 5 RBC aggregation studies showing the absence of aggregation in PIAC-P-based (b) and PIAC-F-based (c) hydrogel scaffolds in comparison with saline control (a) and positive control polyethyleneimine (d).
For aggregation studies, 1 ml of bacterial culture was added to 10 ml aggregation buffer which consisted 20 mM Tris HCl (pH 7.8), 0.01 mM MgCl2, 0.1 M NaCl, and 0.02% sodium azide.
