Objective analysis of LC aggregation revealed a significant decrease in total LC cluster number per testis section in animals exposed to either 100 or 500 mg/kg DBP.
The formation of the autophagosome was monitored by endogenous microtubule-associated protein 1 light chain 3 (LC3) aggregation revealed by staining with a specific LC3 antibody.
In contrast, although the SOD1 can associate with DNA under neutral conditions [47], any observable aggregation phenomenon under the TEM is not found without Asc, as indicated with DSL in examining the time courses of aggregation, revealing that Asc-induced SOD1 oxidation is a key factor in the enhanced aggregation propensity of WT SOD1.
Previous mechanistic studies of TTR aggregation revealed that intermolecular interactions between strands H play a key role in TTR amyloid formation46,47.
Indeed, a quantitative assay for cell aggregation revealed that rTFs with VP64 were only 1.03- to 1.07-fold more effective in generating a signal than those with VP16 (0.1
Our study on the effect of a stereochemical modification on aDrs aggregation revealed interesting aspects of superstructural organization and polymorphism, which is in the focus of current research.
In contrast to the control individuals, the combined application of the tau-antibody AT270 [ 15], as axonal marker, and the rabbit anti-ataxin-3 antibody [ 29], as a marker of ataxin-3 protein aggregation, revealed intensively labeled intra-axonal inclusion bodies in all seven SCA3 patients (Fig. 1; Table 3).
A variety of salts and solutes were screened to determine their influence on 1018 aggregation, revealing that this peptide "salts out" of solution in an anion-specific and concentration-dependent manner.
A parallel analysis of modifiers of polyglutamine aggregation revealed that these modifiers functioned mainly in the cytosol and in the nucleus, indicating that there are very different ways in which cells deal with misfolded polyglutamine proteins or misfolded α-synuclein (Van Ham et al, 2008).
Our combined analyses of the intermediate states formed at the early stage of aggregation revealed molecular details of aggregation pathways toward the formation of distinct cytotoxic TTR oligomers.
Spherical silver nanoparticles can be seen on the surface of ramie fibers, without visible aggregation, revealing silver nanoparticles were formed through reduction of silver ions by ramie fibers.
