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A previous study elucidated that small-sized platelet aggregates ultimately developed into medium-sized and large-sized aggregates as platelet aggregation proceeds.
Along all the temperatures investigated, aggregation proceeds by forming intermediate molecular assemblies that successively aggregate in clusters.
As aggregation proceeds, these differences in the morphology of Josephin aggregates formed in the absence and in the presence of Ub are consistently observed, as shown by EM micrographs obtained after 6 d at 37°C (Fig. 4 C).
Although the misfolding studies under the non-physiological conditions have provided valuable insights into the TTR misfolding pathways33, it is unclear whether TTR aggregation proceeds by the similar oligomerization mechanism at physiologically relevant conditions.
Given a set of shared regions, the aggregation proceeds as in Algorithm 1.
This is affected by the concentration and type of accelerator and ultimately whether the aggregation proceeds according to RLCA or DLCA mechanisms [53, 55, 58].
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Only when this intermediate of aggregation is significantly populated does the process of aggregation proceed to formation of large aggregates.
In contrast, α1 became inhibitory after aggregation proceeded for approximately 6 hr.
Platelet aggregation proceeded faster in the presence of AGE and SHS.
Consistent with previously published data (Tam et al., 2009), mutant huntingtin aggregation proceeded rapidly and reached saturation 10 h after initiation of AcTEV™ protease cleavage of the GST tag (Supplementary Fig. 8A).
In order to determine at what concentration protein aggregation is induced by the varying salt solution conditions, the protein hydrodynamic radius was tracked using DLS measured particle size under identical buffer conditions (although not all conditions were replicated as at some of the higher ionic strengths aggregation proceeded too rapidly to accurately measure using DLS).
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